vpr [2018-09-13 18:11:23]

minor extracellular serine protease

Locus: BSU38090

Isoelectric point: 5.77

Molecular weight: 85.42 kDa

Protein length: 806 aa

Gene length: 2418 bp

Function: protein degradation

Product: minor extracellular serine protease

Essential: no

Synonyms: ipa-45r

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of proteins
• Information processing → Protein synthesis, modification and degradation → Proteolysis → Extracellular feeding proteases
• Groups of genes → Secreted proteins

Gene

Coordinates: 3,907,844 → 3,910,264

The protein

Protein family

• peptidase S8 family (according to Swiss-Prot)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: vpr
  Description: PubMed

  Sigma factors

  • SigH: sigma factor, PubMed, in SigH regulon

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • PhoP: activation, PubMed, in PhoP regulon
  • LexA: repression, PubMed, in LexA regulon
  • DnaA: repression, PubMed, in DnaA regulon

  Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed

Biological materials

Mutant

• KO7 (ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr), available as BGSC 1A1133
• BKE38090 (Δvpr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT, downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT
• BKK38090 (Δvpr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT, downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT

References

Reviews

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Original publications

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